SP5490 美国戴安Dionex AminoPac PA10 Amino Acid Column 生物色谱柱

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产品名称: SP5490 美国戴安Dionex AminoPac PA10 Amino Acid Column 生物色谱柱

产品型号: SP5490

产品展商: 戴安Dionex

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简单介绍

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SP5490 美国戴安Dionex AminoPac PA10 Amino Acid Column 生物色谱柱 的详细介绍

AminoPac PA10 Amino Acid Column

For High-Resolution Separations of Free Amino Acids by AAA-Direct

The AminoPac^® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct^™ Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.

Derivatization not required
Carbohydrates and amino acids separated and detected simultaneously
Mid-femtomole to low-picomole detection limits
Compatible with all commonly used hydrolysis procedures
Can be configured for on-line analysis

AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths.

The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct Specifications
Recommended operating temperature	30 °C
Typical operating conditions	0.25 mL/min gradient Recommended flow rate: 0.25 mL/min
Mobile phase compatibility	pH 0–14; 100% compatible with common organic solvents. Dionex sodium acetate ionic form eluents only.
Resin composition	8.5-µm-diameter microporous (<10 Å) bead (55% cross-linked) agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked).
Anion-exchange capacity	60 µeq/column (2 × 250 mm analytical column)
Column construction	PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.
Dimensions	AminoPac PA10 Analytical Column: 2 × 250 mm AminoPac PA10 Guard Column: 2 × 50 mm
Maximum operating pressure	4000 psi (27.9 MPa)

AminoPac PA10 - Analytical Columns
AminoPac PA10 Analytical Column (2 x 250 mm)	055406
AminoPac PA10 (22 x 250 mm)	SP5488
AminoPac PA10 (9 x 250 mm)	SP5490
AminoPac PA10 Analytical Column (4 x 250 mm)	SP5678

Guard Columns
AminoPac PA10 Guard Column (2 x 50 mm)	055407
AminoPac PA10 Guard Column (4 x 50 mm)	SP5680

Accessories
AAA-Direct Installation Kit	059539
Carbohydrate Removal Cartridge (CRC) for AAA-Direct	068598

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